Phase II — In-Vessel Composting
Phase II Substrate Production — The Science of High-Yield Mushroom Composting
Phase II is the critical “refinement” stage of mushroom substrate production. While Phase I focuses on the initial structural breakdown of raw materials, Phase II is where the science of bioprocessing takes over to create a clean, nutrient-rich, and selective growth medium.
At CNC Substrates, our Indoor Fresh Compost (IVC®) methodology at Milsbeek and Moerdijk represents the global benchmark for this process, moving substrate preparation from an “outdoor art” to an “indoor science.”

The IVC® Advantage — Total Climate Control
Traditional composting is often at the mercy of the elements.
Our IVC® system decouples production from weather variables by moving the process into enclosed, high-tech climate-controlled tunnels. This ensures 100% homogeneity across every ton of substrate, providing growers with a predictable foundation for their crop, regardless of the season.
The Two Pillars of Phase II
The transformation within our Phase II tunnels is driven by two strictly controlled thermal stages:
Precision Pasteurization (60°C)
Safety and biosecurity are the priorities here.
By maintaining a steady peak temperature of 60°C, we effectively eliminate harmful pathogens, pests, and competitive “weed” fungi.
This “kill step” ensures a sterile environment that allows your mushroom mycelium to grow without competition.
Scientific Conditioning (48°C)
Once pasteurized, the substrate undergoes biological conditioning.
During this phase, we manage airflow and temperature to facilitate the conversion of toxic ammonia into nutrient-rich microbial protein.
This process removes the “sharp” smell of ammonia and replaces it with the sweet, earthy aroma of high-quality compost.
Creating a Biological Shield
Phase II is not just about cleaning the substrate; it is about fostering life.
Our methodology promotes the growth of beneficial thermophilic microflora, such as S. thermophilum.
These “good” microbes act as a biological shield, protecting the substrate from future infections while actively stimulating the growth of the Agaricus mycelium.
Environmental Stewardship — Ammonia Capture
Perhaps the most significant technical advancement of the IVC system is its management of ammonia (NH3).
Ammonia is a natural by-product of the breakdown of nitrogenous compounds (urine, urea, poultry manure) during composting.
In open systems, this ammonia volatilizes into the atmosphere, representing a significant source of air pollution and a violation of increasingly strict EU environmental regulations (such as the Nitrate Directive).
CNC Substrates’ IVC facilities are equipped with industrial-scale acid scrubbers and ammonia washers.
The exhaust air from the tunnels is forced through these scrubbers, where the ammonia is captured and converted into ammonium sulfate or similar nitrogenous salts.
This has two profound benefits:
Regulatory Compliance
It allows CNC to operate large-scale facilities like Milsbeek and Moerdijk without exceeding emission caps, ensuring business continuity in a regulated environment.
Nitrogen Efficiency
By capturing the nitrogen rather than letting it gas off, the process retains more nutritional value within the biomass system (or recovers it for other uses), leading to a more efficient use of the raw inputs.
Ready to Optimize Your Agaricus Mushroom Yields?
By mastering the thermal dynamics and microbial ecology of the composting process, we provide a substrate that is:
- Highly Selective
- Pathogen-Free
- Consistent
High-quality harvests begin with a sterile, energy-dense foundation.